that the prosecution’s own sampling and chain of custody procedure was afflicted by multiple departures from the relevant Standard Operating Procedures (“SOP”). [12] SD1 is a technician with the respondent. According to SD1, he collected samples of effluent from the same final discharge point of the respondent’s effluent treatment plant contemporaneously with the DOE’s official sampling. Those samples were subsequently forwarded to two independent private laboratories, namely Nabbir Laboratory (KL) Sdn Bhd (“Nabbir”) and UITM-A&A Laboratory (“A&A”). [13] SD1 acknowledged during cross-examination that he had not performed any preservation of the samples at the point of collection and before their transfer to containers brought by SD4 of Nabbir on 15.03.2021 and to SD6 of A&A who collected the samples on 16.03.2021. [14] SD2 gave evidence that the respondent did not use cadmium in its manufacturing processes. As the Global Facilities Engineer of the respondent, SD2 was personally responsible for supervising daily system monitoring and ensuring compliance with effluent discharge conditions at the respondent’s plant. [15] SD2’s evidence was supported by documentary exhibits including the respondent’s Chemical Register, analysis reports by SGS for 2019 and 2020, chemical analyses of the respondent’s influents and effluents from 2017 to 2021 and studies conducted under the Industrial Effluent Control Scheme (“IECS”) in 2017, 2020 and 2021. [16] The respondent’s compliance with the Restriction of Hazardous Substances (“RoHS”) directives, which restrict the use of Cadmium in electronic manufacturing, was also in evidence. SD2 explained that Cadmium is typically used in printed circuit board or electronic component manufacturing and is not used by semiconductor manufacturers such as the respondent. [17] The results from the two independent private laboratories diverged substantially from those of the Chemistry Department. The analysis by Nabbir detected no Cadmium whatsoever in the samples submitted by the respondent. This was confirmed by SD7 and SD3. [18] The analysis by A&A detected Cadmium in the samples only at a concentration of 0.002 mg/L, a level that did not exceed the prescribed Standard B limit. This was confirmed by SD8 and SD5. The concentration of Cadmium detected by A&A was some five hundred and twenty times lower than the Chemistry Department’s result of 1.04 mg/L. [19] Notably, SD8 who is a chemist with thirty years of experience, gave expert evidence to the effect that heavy metals, including Cadmium, are inherently very stable chemical elements that do not dissipate, vapourise or degrade significantly over time. SD8 testified that heavy metals are stable for up to six months under the APHA analytical method, and that even where preservation is not immediately applied, a Cadmium concentration as high as 1.04 mg/L would remain substantially detectable and could not be reduced to trace levels merely by a delay in preservation of one day. It is important to observe that SD8 specifically said that the deviation in results could not be of such a magnitude even if the sampling procedure had been incorrectly followed. [20] SD5 who is a chemist with seven years of experience from Nabbir Laboratory similarly testified that the degree of deviation between the Chemistry Department’s result and the results of the defence’s laboratories could not be attributable to any non-compliance with the sampling SOP. According to SD5, the deviation would not be that much even if the sampling had been done incorrectly. [21] The learned counsel also raised specific challenges to the prosecution’s own compliance with its sampling SOP. It was in evidence that SP1 failed to record or measure the temperature of the DOE’s official effluent samples in the field or upon delivery to the Chemistry Department, contrary to the requirements of the applicable Effluent SOP. [22] No photographic evidence was adduced to corroborate SP1’s claim that he had used an ice box or purchased ice to maintain the temperature of the samples during transport. SP1 also failed to record or measure the pH of the official samples in the field, and his evidence that he used litmus paper to measure the pH of the sample was unsupported by any documentary or photographic record. [23] SP2, the Government Chemist, claimed that the temperature and pH of the samples were checked at the Chemistry Department and the equipment was in good working order. However, these claims were not supported by any contemporaneous documentation. [24] The prosecution declined to produce the relevant SOP applicable to the DOE and the Chemistry Department’s procedures despite SP1’s acknowledgment that the SOP was very important. There was also a delay in the transfer of the DOE’s official samples to the Chemistry Department which were collected at approximately 10:30am and delivered to the Chemistry Department at 2:37pm. [25] At the conclusion of the defence case, the Sessions Court found that the defence had successfully raised a reasonable doubt in the prosecution’s case by reason of the issue of whether the respondent used Cadmium at all, the results of the heavy metals analysis by A&A Laboratory as confirmed by SD8, and the issues relating to sample temperature, pH, storage conditions and the equipment used by the prosecution’s chemist in conducting the analysis. Accordingly, the Sessions Court acquitted and discharged the respondent. Dissatisfied with that decision, the Public Prosecutor lodged the present appeal. Analysis and Findings Whether the respondent used cadmium in its manufacturing processes [26] The first substantive contention advanced by the learned DPP is that the respondent’s position that it did not use Cadmium in its manufacturing processes is unsubstantiated. The learned DPP submitted, with reference to the Chemistry Department’s analysis and A&A’s confirmation that trace quantities of Cadmium were detected and hence the contention of non-use of Cadmium is therefore contradicted by the evidence of both sides. [27] The learned DPP further submitted that the evidence of SP1 and SD2 to this effect constituted mere personal opinion unsupported by proof. The learned DPP specifically argued that the evidence of SP1, an investigating officer who confirmed under cross-examination that he was aware the respondent did not use Cadmium, and SD2, the respondent’s engineer, should be dismissed as individual opinions not grounded in documentary proof. [28] Respectfully, I am unable to agree. SP1 is the prosecution’s own investigating officer, a person who had access to DOE’s records pertaining to the respondent as part of his investigation. SP1 testified during cross-examination that his knowledge that the respondent did not use Cadmium was derived from his review of the company’s source records held on DOE’s files and from DOE’s Online Environmental Reporting system. [29] SP1 is not merely any member of the public expressing a personal view. He is the DOE’s own officer who reviewed the very records that the DOE maintains on regulated premises. SP1’s concession on this point is not, as the learned DPP contended, a mere personal opinion. It is an acknowledgment grounded in official regulatory records and was therefore of significant evidential weight. [30] SD2’s evidence was equally substantiated. She was the Global Facilities Engineer responsible for supervising and monitoring all water-related processes at the respondent’s plant, including ensuring compliance with effluent discharge conditions. SD2 had personal and direct knowledge of the chemical inputs used in the respondent’s manufacturing processes. Her evidence was corroborated by an extensive body of documentary proof, including the respondent’s Chemical Register, the results of periodic chemical analysis of the respondent’s influents and effluents from 2017 to 2021 showing no Cadmium at significant levels, analysis reports from SGS for 2019 and 2020 and IECS studies over multiple years. [31] The respondent’s compliance with RoHS directives further supported this position. The characterisation of this evidence as “personal opinions unsupported by proof” is unsustainable on the record and I am unable to agree with such a posit. [32] In so far as A&A detected Cadmium at a concentration of 0.002 mg/L, the respondent offered an explanation that this minute quantity may have originated from external sources such as piping or surface run-off rather than from the respondent’s manufacturing processes. SD8 and SD2 both gave evidence to this effect, noting that trace quantities of Cadmium can enter effluent streams from various incidental sources. [33] This explanation was not effectively rebutted by the prosecution and is consistent with the overall evidence that the respondent did not use Cadmium as an input material in its processes. On the evidence, it is the finding of this Court that this issue was therefore correctly resolved by the Sessions Court in favour of the respondent. Whether the A&A laboratory results were unreliable by reason of non-compliance with the sampling SOP [34] This was the most extensively argued ground of appeal. The learned DPP contended that the samples collected by SD1 on 15.03.2021 were not immediately preserved in accordance with the applicable SOP and that this failure fatally undermines the reliability of the A&A laboratory results. The learned DPP relied on SD1’s own admission that no preservation was performed at the point of collection, on the SOP’s requirement that preservation be added as soon as samples are collected, and on the evidence of SD5 and SD8 that non-preservation can affect the accuracy of analytical results by causing an adsorption effect and unreliable pH readings. The learned DPP argued that A&A’s results of 0.002 mg/L should therefore be disregarded and submitted that the prosecution has proved its case by the Chemistry Department’s result alone. [35] Respectfully, this Court is unable to accept this argument for several reasons, which are now addressed in turn. [36] First and most fundamentally, the very expert witnesses whose evidence the learned DPP relied upon for the proposition that non-preservation affects analytical reliability themselves provided critical evidence that the degree of difference between the Chemistry Department result and the A&A result could not be explained by any failure to preserve. [37] SD8 gave evidence that heavy metals, including Cadmium, are inherently very stable elements that do not vanish, vapourise or degrade substantially over time. SD8 testified unequivocally that Cadmium at a concentration as high as 1.04 mg/L would remain substantially present in a sample even if that sample were not immediately preserved as heavy metals are classified as stable parameters that can be analysed validly within a window of up to six months under the APHA method. SD8 testified that even if the sampling procedure was incorrectly followed, the deviation in results would not be of the magnitude seen between 1.04 mg/L and 0.002 mg/L, and that it was highly unlikely that a concentration of 1.04 mg/L could be reduced to a minute trace of 0.002 mg/L merely by a single day’s delay in the application of preservatives. [38] SD5 similarly testified that the deviation between the Chemistry Department’s result and the independent results would not be of such magnitude even if the sampling procedure had been done incorrectly. [39] This evidence was of considerable importance. It was not merely the respondent asserting that the A&A results were accurate. It is the respondent’s own expert witnesses providing a principled scientific explanation for why the magnitude of the discrepancy cannot be attributed to any sampling non-compliance. Pertinently, this evidence was not effectively challenged by the prosecution. [40] The learned DPP’s argument in effect asked this Court to selectively accept part of the expert evidence, namely the general principle that preservation is important, while ignoring the direct expert evidence that the scale of the discrepancy between 1.04 mg/L and 0.002 mg/L cannot be explained by any non-compliance with preservation protocols. The Court is unable to agree. [41] Second, as the respondent pointed out, the samples that SD6 collected from SD1 for A&A on 16.03.2021 were placed in containers that had already been preserved by A&A laboratory staff before being brought to the respondent’s premises. This meant that the samples were not unpreserved for an indefinite period. They were placed into pre-preserved containers on the day following collection. [42] Given SD8’s evidence that Cadmium is a very stable element that does not degrade within such a short timeframe, it is highly improbable that the one-day interval between collection and preservation had any material effect on the analytical results. [43] Third, the learned DPP’s argument on sample integrity, if accepted, carries logical implications that extend to the prosecution’s own samples. The learned DPP urged the Court to accept that non-compliance with preservation SOP invalidated the defence’s samples, but it simultaneously asked the Court to treat the prosecution’s samples as reliable without establishing that those samples complied with the applicable SOP. [44] It is pertinent to observe that the prosecution’s SOP compliance in respect of the official samples was itself called into question by the evidence adduced during the defence case. This brings us to the next issue. Whether the prosecution’s own sampling and chain of custody was free from deficiency [45] A separate but related ground raised by the respondent in resisting the appeal concerned the integrity of the prosecution’s own sampling and chain of custody procedure. [46] The learned DPP submitted at trial and on appeal that the official samples collected by the DOE were handled correctly and that SP2’s analysis at the Chemistry department was conducted using a properly calibrated atomic absorption spectrometer (“AAS”) in accordance with recognised analytical methods. However, the defence placed a detailed challenge to the prosecution’s compliance with its own SOP which the prosecution declined to produce at trial. [47] The respondent pointed to the following specific procedural deficiencies in the prosecution’s handling of its official samples: